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G. Rajender ¹, G. Benarjee ¹, M. S. K. Prasad ¹, B. Laxma Reddy ¹, B. Narayana Rao ¹

Affiliations
1 Dept of Zoology, & Dept of Bio- chemistry, Kakatiya University, Warangal-506009, IN

Abstract

Riboflavin binding protein (Rfbp) was isolated from domestic fowl (Gallus gallus) and peahen (Pavo cristatus) egg-white and egg-yolk. The protein was purified in two steps, DEAE-Sephadex A-50 ion exchange chromatography and eluted with phosphate buffer pH 7.3 containing 0.5 M sodium chloride. The final purification of protein was achieved on Sephadex G-100. The purity of the protein was judged on cylindrical and slab gel electrophoresis, SDS-PAGE technique. Sephadex G-100 fraction Rfbp moved as a single band both on the Slab and Cylindrical gels. Comparison of the mobility of Rfbp with that of the standard molecular weight marker proteins revealed with that the Rfbp had a molecular weight close to 29,000 kd. Interestingly, hen egg-white Rfbp and peahen egg- white, yolk Rfbp had the same molecular weight as revealed by the SDS-PAGE. This is a novel approach for the study of riboflavin binding protein purified from different avian eggs in two steps and studied electrophoretic characterization with standard molecular weight marker.

Keywords

Rfbp Purification Method, Peahen, Hen, Egg White-yolk, SDS, PAGE/native

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Ramchander Merugu ¹, M. S. K. Prasad ¹, D. Vasavi ², S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

Four anoxygenic phototrophic bacteria, Rhodobacterium (Rb.) capsulatus, Rhodopseudomonas (Rps.) acidophila, Rps. rutila and Rhodospirillum (Rsp.) rubrum, isolated from wastewaters, were tested for their ability to solubilize tricalcium and dicalcium phosphate under different conditions. Rps. rutila was superior to other bacteria under investigation in solubilisation of tricalcium phosphate. Rsp. rubrum was poor in solubilization of tricalcium phosphate. Rsp. rubrum and Rb. capsulatus could solubilise dicalcium phosphate with almost same efficiency while Rps. acidophila was more efficient in solubilization of dicalcium phosphate. Thus, these bacteria can be exploited in solubilization of phosphate.

Keywords

Rb. capsulatus, Rps. acidophila, Rps. rutila, Rsp. rubrum, Phosphate Solubilization, Dicalcium Phosphate, Tricalcium Phosphate.

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Ramchander Merugu ¹, M. S. K. Prasad ¹, S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

The tolerance of some pesticides by two anoxygenic phototrophic bacteria, isolated from leather industry effluents, was investigated. Rhodobacterium capsulatus was found to be more resistant than Rhodopseudomonas acidophila to the pesticides tried in the present investigation. The pattern of inhibition of growth for both the organisms show that chlorpyrifos was most potent followed by methyl parathion, endosulfan, rogor and kitazin in a descending order.

Keywords

Pesticides, Biofertilizers, Rb. capsulatus, Rps. Acidophila, Leather Industry Effluent.

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Ramchander Merugu ¹, M. S. K. Prasad ¹, S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

The presence of photosynthetic bacteria along with the heterotrophic bacteria has been reported in various aquatic environments like Indian tropical waters, salt marshes, industrial effluents, seawater, sewage water and hot water springs. Two anoxygenic phototrophic bacteria Rb. capsulatus and Rps. acidophila were isolated from leather industrial effluents and influence of some heavy metals on the growth of these two bacteria was studied. Iron and cadmium requirement for the growth of Rb. capsulatus and Rps. acidophila was very low, while selenium and cerium failed to influence the growth of both the bacteria under investigation. Rps. acidophila was highly sensitive to and was totally inhibited by mercuric chloride at a very low concentration, whereas Rb. capsulatus exhibited considerable resistance. Biological significance of above observations in the light of existing literature is discussed.

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B. Satheesh Kumar ¹, M. Krishna Reddy ², M. S. K. Prasad ¹, A. V. N. Appa Rao ³

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal (A.P.), IN
2 Department of Zoology, University College, Kakatiya University, Warangal (A.P.), IN
3 University College of Pharmaceutical Sciences, Kakatiya University, Warangal (A.P.), IN

Abstract

Progesterone is important for initiation and maintenance of pregnancy (hormone of pregnancy).The discovery that in the absence of progesterone, pregnancy cannot be initiated or maintained provided the basis for a massive effort of synthesis of antiprogestin molecules to develop emergency contraceptive pills. By the consequences, till date few progesterone antagonists (antiprogestins) have been synthesized which bind to and block the progesterone receptor. However, none of the antiprogestins are “pure” antiprogestins, having marked antiglucocorticoid properties as well, especially at higher doses. Thus the search for the “ideal” progesterone antagonist continues. The main objective of the present work is to evaluate antiprogestin activity of progesterone analogue, 14 β- hydroxy progesterone. A pregnane glycoside, carumbelloside-I (3-O-beta-D-glucopyranosyl-(1→6)-beta-Dglucopyranosyl- 3beta,14beta-dihydroxypregn-5-en-20-one) was isolated from the n-butanolic extract of Caralluma umbellata (Asclepiadaceae) and was modified to a structural analog of progesterone, 14 β- Hydroxy progesterone (14β- OHP) in just two simple steps. Anti-implantation and abortifacient activities of the compound have been studied to evaluate its antiprogestin activity. To study the anti-implantation effect, pregnant wistar strain albino rats were administered with 14β-OHP (daily dose of 5 mg/Kg body weight) intraperitoneally from the day 1to day 7 of pregnancy. On day 10, laparotomy was performed under light ether anesthesia. The uteri were examined to determine the number of implantation sites. The abdomens were sutured and the animals were allowed to go on term. The number of young born at term was also recorded. The abortifacient activity was studied in another group of pregnant rats. The animals were treated with 14β- OHP (5 mg/Kg body weight daily) intraperitoneally from day 8 to 12of pregnancy. They were sacrificed on day 19 of pregnancy. Both horns of uterus were observed for the number of live fetuses. The compound showed about 44.23% anti-implantation and 62% of abortifacient effect at their corresponding dose levels tested. The results indicated the antiprogestin activity of 14 β- hydroxy progesterone in albino rats.

Keywords

14β- OHP, Anti-Implantation, Abortifacient, Antiprogestin and Contraceptive.

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R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ²

Affiliations
1 Department of Biochemistry, NRI Medical College, China Kakani (A.P.), IN
2 Department of Chemistry, Kakatiya University, WARANGAL (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) from pigeon egg white and egg yolk have been isolated. The molecular weight of purified RCP of pigeon egg white and yolk has been found to be nearly same as that of previously isolated hen egg white and yolk RCPs. The amino acid composition of pigeon RCPs were observed to be almost similar to that of hen RCPs.

Keywords

Riboflavin Carrier Protein, Amino Acid Composition, Purification, Columba livia.

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R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ³

Affiliations
1 Department of Biochemistry, N. R. I. Medical College, CHINA KAKANI, (A.P.), IN
2 Department of Chemistry, Univ. Arts & Science College, Kakatiya University, Warangal, (A.P.), IN
3 Department of Chemistry, Kakatiya University, Warangal, (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) has been purified from both pigeon and hen egg- yolk by ion exchange chromatography and gel filtration techniques. Purity of pigeon egg- yolk and hen egg-yolk RCPs are determined by SDS-PAGE. Their structures are compared using Circular Dichroism (cd) Spectra and Fluorescence Spectra. For Pigeon egg-yolk RCP amino acid composition was analyzed. In the present study it is observed that the two proteins have similar secondary structure but they differ in their tertiary structures. The amino acid composition of pigeon egg-yolk RCP has close similarity with hen egg-yolk RCP. This study suggests the existence of some significant structural difference between RCPs from pigeon and hen.

Keywords

Riboflavin Carrier Protein, Protein Purification and Characterization.

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